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An Artificial Amphiphilic Peptide Promotes Endocytic Uptake by Inducing Membrane Curvature
journal contribution
posted on 2020-05-13, 12:03 authored by Toshihiro Masuda, Hisaaki Hirose, Kentarou Baba, Astrid Walrant, Sandrine Sagan, Naoyuki Inagaki, Toyoshi Fujimoto, Shiroh FutakiMembrane
curvature plays a pivotal role in cellular life, including
cellular uptake and membrane trafficking. The modulation of membrane
curvature provides a novel means of manipulating cellular events.
In this report, we show that a nine-residue amphiphilic peptide (R6W3)
stimulates endocytic uptake by inducing membrane curvature. Curvature
formation on cell membranes was confirmed by observing the cellular
distribution of the curvature-sensing protein amphiphysin fused with
a yellow fluorescent protein (Amp-YFP). Dot-like signals of Amp-YFP
were visible following the addition of R6W3, suggesting curvature
formation in cell membranes, leading to endocytic cup and vesicle
formation. The promotion of endocytic uptake was confirmed using the
endocytosis marker polydextran. Treatment of cells with R6W3 yielded
a 4-fold dextran uptake compared with untreated cells. The amphiphilic
helical structure of R6W3 was also crucial for R6W3-stimulated endocytic
uptake.
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cell membranesamphiphilic helical structureInducing Membrane Curvature Membrane curvatureendocytic uptakeR 6W endocytic uptakemembrane curvaturenine-residue amphiphilic peptideAmp-YFPendocytosis marker polydextranR 6WformationArtificial Amphiphilic Peptide Promotes Endocytic Uptakecurvature-sensing protein amphiphysin
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