ja0c01365_si_001.pdf (1.85 MB)
Aminopeptidase N Activatable Fluorescent Probe for Tracking Metastatic Cancer and Image-Guided Surgery via in Situ Spraying
journal contribution
posted on 2020-03-19, 21:13 authored by Haidong Li, Qichao Yao, Wen Sun, Kun Shao, Yang Lu, Jeewon Chung, Dayeh Kim, Jiangli Fan, Saran Long, Jianjun Du, Yueqing Li, Jingyun Wang, Juyoung Yoon, Xiaojun PengThe recurrence of
malignant tumors is mostly caused by incompleted
surgical resection. Especially, it is difficult for surgeons to detect
and accurately remove metastatic tumors by predominantly using visual
examination and palpation owing to the lack of effective means to
specifically distinguish the boundary range between normal and tumor
tissues. Thus, the development of activated fluorescent probe with
superior tumor-to-normal (T/N) tissue ratios is particularly urgent
in clinics. In view of CD13/aminopeptidase N (APN) regarded as a cancer-specific
biomarker, mediating with progression, invasion, and migration of
malignant tumor, herein, we reported an APN-responsive fluorescent
probe YH-APN and demonstrated its application to distinguish cancer
cells. Through in situ spraying manner, fluorescent
superior tumor-to-normal (T/N) tissue ratios (subcutaneous transplantation
tumor, 13.86; hepatic metastasis, 4.42 and 6.25; splenic metastasis,
4.99) were achieved. More importantly, we have demonstrated the ability
to image metastasis tumor tissue less than 1 mm in diameter, highlighting
the potential for this probe to be used as a tool in surgical resection.
This research may spur the use of enzyme-activatable fluorescent probes
for the progress of tumor diagnosis and image-guided surgery (IGS).