ab7b00645_si_001.pdf (2.38 MB)
Alternative SiO2 Surface Direct MDCK Epithelial Behavior
journal contribution
posted on 2017-10-12, 00:00 authored by Alan D. Covell, Zheng Zeng, Taylor Mabe, Jianjun Wei, Amy Adamson, Dennis R. LaJeunesseThe
mechanical interactions of cells are mediated through adhesive
interactions. In this study, we examined the growth, cellular behavior,
and adhesion of MDCK epithelial cells on three different SiO2 substrates: amorphous glass coverslips and the silicon oxide layers
that grow on ⟨111⟩ and ⟨100⟩ wafers. While
compositionally all three substrates are almost similar, differences
in surface energy result in dramatic differences in epithelial cell
morphology, cell–cell adhesion, cell–substrate adhesion,
actin organization, and extracellular matrix (ECM) protein expression.
We also observe striking differences in ECM protein binding to the
various substrates due to the hydrogen bond interactions. Our results
demonstrate that MDCK cells have a robust response to differences
in substrates that is not obviated by nanotopography or surface composition
and that a cell’s response may manifest through subtle differences
in surface energies of the materials. This work strongly suggests
that other properties of a material other than composition and topology
should be considered when interpreting and controlling interactions
of cells with a substrate, whether it is synthetic or natural.
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protein expressionMDCK Epithelial BehaviorMDCK epithelial cellsepithelial cell morphologyMDCK cellsAlternative SiO 2 Surfaceadhesionactin organizationsurface energy resultsurface compositionsurface energiessilicon oxide layershydrogen bond interactionsextracellular matrixECM protein bindingglass coverslipsSiO 2 substrates
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