nn7b07439_si_003.avi (4.64 MB)
Aligned Carbon Nanotubes Reduce Hypertrophic Scar via Regulating Cell Behavior
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posted on 2018-07-24, 00:00 authored by Weizong Weng, Sisi He, Hongyuan Song, Xiaoqun Li, Liehu Cao, Yajie Hu, Jin Cui, Qirong Zhou, Huisheng Peng, Jiacan SuHypertrophic
scars, characterized by excessive cell proliferation,
disordered cell growth, and aberrant deposition of collagens, could
cause significant clinical problems. Herein, aligned carbon nanotubes
(ACNTs) were synthesized via chemical vapor deposition,
and bulk ACNTs were pulled out from the arrays. The capacity of the
ACNTs to reduce hypertrophic scar formation was evaluated both in vitro and in vivo. The results demonstrated
that the ACNTs suppressed the overproliferation of fibroblast cells,
directed their growth, and inhibited collagen expression in
vitro without cell cytotoxicity. Moreover, in vivo evaluation in a rabbit ear model indicated relieved scar hypertrophy
after the ACNTs treatment. The gene expression microarray was further
used to understand the mechanism, which showed that ACNTs could inhibit
the TGFβ pathway to alter the components in the extracellular
matrix, cell proliferation, cell cytoskeleton, and cell motility.
These findings may provide a potent strategy of using carbon nanotubes
in the bioengineering field.