bm500199h_si_001.pdf (14.24 MB)
Aggregation Behavior of Cationic Nanohydrogel Particles in Human Blood Serum
journal contribution
posted on 2014-04-14, 00:00 authored by Lutz Nuhn, Sabine Gietzen, Kristin Mohr, Karl Fischer, Kazuko Toh, Kanjiro Miyata, Yu Matsumoto, Kazunori Kataoka, Manfred Schmidt, Rudolf ZentelFor
systemic siRNA delivery applications, well-defined drug carriers
are required that guarantee stability for both carrier and cargo.
Among various concepts progressing in market or final development,
cationic nanohydrogel particles may serve as novel transport media
especially designed for siRNA-in vivo experiments. In this work, the
interaction of nanohydrogel particles with proteins and serum components
was studied via dynamic light scattering in human blood serum as novel
screening method prior to applications in vivo. The formation of larger
aggregates mostly caused by charge interaction with albumin could
be suppressed by nanogel loading with siRNA affording a neutral zeta
potential for the complex. Preliminary in vivo studies confirmed the
results inside the light-scattering cuvette. Although both carrier
and cargo may have limited stability on their own under physiological
relevant conditions, they can form safe and stable complexes at a
charge neutralized ratio and thus making them applicable to systemic
siRNA delivery.
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nanogel loadingnovel screening methodAggregation Behaviorvivo studiesnanohydrogel particlescargoHuman Blood SerumForcharge interactionsiRNA delivery applicationsdrug carriersnovel transport mediasiRNA deliveryCationic Nanohydrogel Particlesserum componentsblood serumguarantee stabilitycationic nanohydrogel particles
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