jf5b02489_si_001.pdf (1.43 MB)
Accurate Analysis and Evaluation of Acidic Plant Growth Regulators in Transgenic and Nontransgenic Edible Oils with Facile Microwave-Assisted Extraction–Derivatization
journal contribution
posted on 2015-09-16, 00:00 authored by Mengge Liu, Guang Chen, Hailong Guo, Baolei Fan, Jianjun Liu, Qiang Fu, Xiu Li, Xiaomin Lu, Xianen Zhao, Guoliang Li, Zhiwei Sun, Lian Xia, Shuyun Zhu, Daoshan Yang, Ziping Cao, Hua Wang, Yourui Suo, Jinmao YouDetermination of plant growth regulators
(PGRs) in a signal transduction
system (STS) is significant for transgenic food safety, but may be
challenged by poor accuracy and analyte instability. In this work,
a microwave-assisted extraction–derivatization (MAED) method
is developed for six acidic PGRs in oil samples, allowing an efficient
(<1.5 h) and facile (one step) pretreatment. Accuracies are greatly
improved, particularly for gibberellin A3 (−2.72
to −0.65%) as compared with those reported (−22 to −2%).
Excellent selectivity and quite low detection limits (0.37–1.36
ng mL–1) are enabled by fluorescence detection–mass
spectrum monitoring. Results show the significant differences in acidic
PGRs between transgenic and nontransgenic oils, particularly 1-naphthaleneacetic
acid (1-NAA), implying the PGRs induced variations of components and
genes. This study provides, for the first time, an accurate and efficient
determination for labile PGRs involved in STS and a promising concept
for objectively evaluating the safety of transgenic foods.