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Accomplished High-Resolution Metabolomic and Molecular Studies Identify New Carotenoid Biosynthetic Reactions in Cyanobacteria
journal contribution
posted on 2020-05-22, 16:36 authored by Mariana
Manzoni Maroneze, Belén Caballero-Guerrero, Leila Queiroz Zepka, Eduardo Jacob-Lopes, Antonio Pérez-Gálvez, María RocaCyanobacteria
and microalgae are characterized by a rich and varied
profile of chlorophyll (8–20 mg/g) and carotenoid (>2.2
mg/g)
pigments, being noteworthy material for natural pigment production
in the food industry. We propose a systematic workflow that uses high-performance
liquid chromatography (HPLC) coupled with high-resolution tandem mass
spectrometry in a broadband collision-induced dissociation mode (bbCID)
acquisition mode to simultaneously obtain MS and MSn spectra. Metabolomic studies showed for the first time
the presence of carotenoids with 5,6-epoxy-groups (5,6-epoxy- and
5,8-furanoid β-cryptoxanthin), carotenoids from the α-branch
(5,8-furanoid-2′-3′-didehydro α-cryptoxanthin),
and 2′-dehydrodeoxomyxol in cyanobacteria. To support the new
findings, an in silico search retrieved the putative
sequences of carotenogenic enzymes involved in the corresponding biosynthetic
pathways (ZEP, NSY, CrtL-b and CrtR) in the analyzed cyanobacteria
species. Consequently, high-throughput metabolomics studies assisted
by molecular analysis offer a powerful tool for providing insights
into the characterization of bioactive compounds and their metabolism
in cyanobacteria and microalgae.
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tandem mass spectrometrycarotenogenic enzymesAccomplished High-Resolution MetabolomicHPLCmgMetabolomic studiesbioactive compoundscyanobacteria speciesCyanobacteria Cyanobacteriahigh-throughput metabolomics studiesfuranoidα-pigment productionMolecular Studies Identify New Carotenoid Biosynthetic Reactionssilico searchcryptoxanthinfood industrybiosynthetic pathwaysZEPacquisition modemicroalgaeanalysis offerNSYbroadband collision-induced dissociation modeMS n spectracarotenoid
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