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A Universal Electrochemical Biosensor Using Nick-HCR Nanostructure as Molecular Gate of Nanochannel for Detecting Chromium(III) Ions and MicroRNA
journal contribution
posted on 2019-10-18, 19:33 authored by Longjiao Zhu, Miao Miao, Xiangli Shao, Zaihui Du, Kunlun Huang, Yunbo Luo, Wentao XuSolid-state
nanochannels demonstrating excellent mechanical properties
and chemical stability combined with programmable DNA provide an opportunity
to control on-demand ion transport. However, poor functionalization
of the nanochannels limits the types of detected targets, as well
as its universality in the sensing field. To solve these issues, a
universal nanochannel sensing platform was developed by employing
a nick hybridization chain reaction (nHCR) nanostructure as a molecular
gate, which could generally respond to the universal sequence Y. Metal
ion-dependent DNAzyme cleavage was used to transfer the chromium(III)
(Cr3+) ions into nucleic acid X, which was further amplified
and converted into universal sequence Y. Upon adding sequence Y into
the nHCR nanostructure-functionalized nanochannel, the disassembly
of the nHCR molecular gate turned on the ionic current signal inside
the nanochannel. The ON–OFF ratio displayed a linear relationship
with the Cr3+ concentration in the range from 200 fM to
20 nM. In less than 66 min, the nanochannel-based biosensing platform
successfully detected Cr3+ ions as low as 200 fM. In addition,
the detection of microRNA with a concentration as low as 1 pM was
achieved by only regulating the sequence of template X′–Y′.