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A Toolkit to Quantify Target Compounds in Thin-Layer-Chromatography Experiments
journal contribution
posted on 2018-10-12, 13:55 authored by Niamh Mac Fhionnlaoich, Stuart Ibsen, Luis A. Serrano, Alaric Taylor, Runzhang Qi, Stefan GuldinThin-layer chromatography (TLC) is
one of the basic analytical
procedures in chemistry and allows the demonstration of various chemical
principles in an educational setting. An often-overlooked aspect of
TLC is the capability to quantify isolated target compounds in an
unknown sample. Here, we present a suitable route to implement quantitative
analysis in a lesson plan. We provide both a stand-alone software
and an online webapp that allow students to obtain quantitative information
from a developed TLC plate and present two suitable experiments, namely,
the absorbance-based quantification of the colorant Sudan IV and the
fluorescence-based quantification of rhodamine 6G, a fluorophore widely
used in biotechnology. Students conduct TLC experiments following
established protocols, take pictures of their TLC plates with
mobile phones, and subsequently quantify the different compounds
in the separate bands they observe.
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rhodamine 6 GTLC plateslesson planabsorbance-based quantificationQuantify Target Compoundsfluorescence-based quantificationStudents conduct TLC experimentsThin-Layer-Chromatography Experiments Thin-layer chromatographycolorant Sudan IVdifferent compoundsTLC plateoften-overlooked aspecttarget compoundschemical principles
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