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A Simple and Effective Sample Preparation Strategy for MALDI-MS Imaging of Neuropeptide Changes in the Crustacean Brain Due to Hypoxia and Hypercapnia Stress
journal contribution
posted on 2020-03-23, 19:03 authored by Amanda
R. Buchberger, Nhu Q. Vu, Jillian Johnson, Kellen DeLaney, Lingjun LiMatrix-assisted
laser desorption/ionization (MALDI)-MS imaging
has been utilized to image a variety of biomolecules, including neuropeptides.
Washing a tissue section is an effective way to eliminate interfering
background and improve detection of low concentration target analyte
molecules; however, many previous methods have not been tested for
neuropeptide analysis via MALDI-MS imaging. Using crustaceans as a
neurological model organism, we developed a new, simple washing procedure
and applied this method to characterize neuropeptide changes due to
hypoxia stress. With a 10 s 50:50 EtOH:H2O wash, neuropeptide
coverage was improved by 1.15-fold, while normalized signal intensities
were increased by 5.28-fold. Specifically, hypoxia and hypercapnia
stress conditions were investigated due to their environmental relevance
to marine invertebrates. Many neuropeptides, including RFamides, pyrokinin,
and cardioactive peptides, showed distinct up- and down-regulation
for specific neuropeptide isoforms. Since crustacean neuropeptides
are homologous to those found in humans, results from these studies
can be applied to understand potential roles of neuropeptides involved
in medical hypoxia and hypercapnia.
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neuropeptide coverageMALDI-MS ImagingMALDI-MS imagingmarine invertebratesneuropeptide analysiscrustacean neuropeptidesmodel organismtissue sectionsignal intensitiesMany neuropeptidescardioactive peptidesneuropeptide isoformshypercapnia stress conditionsneuropeptide changesconcentration target analyte moleculesEffective Sample Preparation Strategyhypoxia stressCrustacean BrainNeuropeptide Changes
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