ac7b03636_si_001.pdf (1.6 MB)
A Novel Color Modulation Analysis Strategy through Tunable Multiband Laser for Nanoparticle Identification and Evaluation
journal contribution
posted on 2018-01-15, 00:00 authored by Xuan Cao, Gang Lei, Jingjing Feng, Qi Pan, Xiaodong Wen, Yan HeCreating
color difference and improving the color resolution in
digital imaging is crucial for better application of color analysis.
Herein, a novel color modulation analysis strategy was developed by
using a homemade tunable multiband laser illumination device, in which
the portions of R, G, and B components of the illumination light are
discretionarily adjustable, and hence the sample color could be visually
modulated continuously in the RGB color space. Through this strategy,
the color appearance of single gold nanorods (AuNRs) under dark-field
microscopy was migrated from the spectrally insensitive red region
to the spectrally sensitive green-yellow region. Unlike the traditional
continuous-wave light source illumination, wherein the small spectral
variations in the samples within a narrow spectral range are averaged
by the whole spectrum of the light source, leading to little color
difference, the application of sharp, multiband laser illumination
could enlarge the color separation between samples, thus resulting
in high spectral sensitivity in color analysis. By comparing the corresponding
color evolution processes of different samples as the multiband combination
of the laser illumination was changed, more efficient color separation
of AuNRs was achieved. With this instrument and single Ag@AuNRs as
the sulfide probe, we achieved high throughput and highly sensitive
detection of sulfide at a detection limit of 0.1 nM, a more than 2
orders of magnitude improvement compared to the previous color sensing
scheme. This strategy could be utilized for nanoparticle identification,
evaluation, and determination in biological imaging and biochemical
analysis.
History
Usage metrics
Categories
Keywords
color separationNovel Color Modulation Analysis Strategymultiband laser illuminationRGB color spacecontinuous-wave light source illuminationtunable multiband laser illumination deviceTunable Multiband Lasercolor differencecolor evolution processesnovel color modulation analysis strategycolor analysis
Licence
Exports
RefWorks
BibTeX
Ref. manager
Endnote
DataCite
NLM
DC