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A Multicolor Immunosensor for Sensitive Visual Detection of Breast Cancer Biomarker Based on Sensitive NADH-Ascorbic-Acid-Mediated Growth of Gold Nanobipyramids
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posted on 2019-12-13, 22:44 authored by Zongwen Wang, Qian Chen, Yingying Zhong, Xinhui Yu, Yongning Wu, FengFu FuMany studies have demonstrated that the extracellular
domain of
human epidermal growth factor receptor 2 (HER2 ECD) level in serum
can act as a breast cancer biomarker and serve as a monitoring neoadjuvant
therapy of breast cancer. In this study, we developed a sensitive
ascorbic acid (AA)-mediated AuNBPs (gold nanobipyramids) growth method
with NADH (reduced nicotinamide adenine dinucleotide I) assistance,
and we further fabricated a high-resolution multicolor immunosensor
for sensitive visual detection of HER2 ECD in serum by using AuNBPs
as signal and antibody as recognition probe. The NADH-assisted AA-mediated
method effectively suppressed color formation in the blank and greatly
improved the sensitivity of mediating AuNBPs growth, allowing us to
use a low concentration of AA to mediate AuNBPs growth to generate
more colorful and clearer color changes. The proposed multicolor immunosensor
has higher resolution and more color changes corresponding to HER2
ECD concentrations. It can be used to detect as low as 0.5 ng/mL of
HER2 ECD by bare eye observation and 0.05 ng/mL of HER2 ECD by UV–visible
spectrophotometry. Using the immunosensor, we have successfully detected
HER2 ECD in human serum with a recovery of 94%–96% and an RSD
(n = 5) < 5%. The results obtained with our immunosensor
were consistent with those obtained with ELISA, verifying the immunosensor
has good accuracy. The immunosensor exhibited a vivid multicolor change,
has low visual detection limit, excellent specificity and reproducibility,
and robust resistance to matrix. All the above features makes our
immunosensor a promising assay for the early diagnosis of HER2-dependent
breast cancers in clinical diagnosis.
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HER 2 ECDHER 2-dependent breast cancersbreast cancer biomarkerAuNBPs growthHER 2 ECD concentrationsnicotinamide adenine dinucleotideUVSensitive NADH-Ascorbic-Acid-Mediated GrowthBreast Cancer BiomarkerELISAmonitoring neoadjuvant therapyepidermal growth factor receptor 2serumcolor changesNADH-assisted AA-mediated methodimmunosensorRSD
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