bi300535a_si_001.pdf (5.17 MB)
A Facile Method to Synthesize Histones with Posttranslational Modification Mimics
journal contribution
posted on 2012-07-03, 00:00 authored by Zhiyong
U. Wang, Yane-Shih Wang, Pei-Jing Pai, William K. Russell, David H. Russell, Wenshe R. LiuUsing an evolved pyrrolysyl-tRNA synthetase-tRNAPyl pair,
a Se-alkylselenocysteine was genetically incorporated
into histone H3 with a high protein expression yield. Quantitative
oxidative elimination of Se-alkylselenocysteine followed
by Michael addition reactions with various thiol nucleophiles generated
biologically active mimics of H3 with posttranslational modifications
including lysine methylation, lysine acetylation, and serine phosphorylation.