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A Facile Colorimetric and Spectrophotometric Method for Sensitive Determination of Metformin in Human Serum Based on Citrate-Capped Gold Nanoparticles: Central Composite Design Optimization
journal contribution
posted on 2019-10-09, 14:33 authored by Neda Shahbazi, Rouholah Zare-DorabeiFor
the determination of Metformin in human serum, a facile colorimetric
and spectrophotometric sensor was designed based on citrate-capped
gold nanoparticles (citrate-GNPs). In this probe, the addition of
Metformin to GNP solution generates a naked-eye color change resulting
from the aggregation of GNPs. Study of this color conversion and quantity
analysis of analyte is operated by spectrophotometric instruments.
The three factors pH, time, and GNP ratio were selected to examine
their effects on sensing results and their values optimization. The
optimization of parameters was done by means of central composite
design and one-at-a-time methods. The sensing results proved the highly
selective and sensitive performance of the sensor for Metformin in
a linear range of 6.25–133.3 ppm with a detection limit of
1.79 ppm. The relative standard deviation (RSD) of the reported method
is 2.53%.
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1.79 ppmGNP solutionquantity analysisdetection limitMetforminone-at-a-time methodsvalues optimizationSensitive DeterminationGNP ratioCentral Composite Design Optimizationcitrate-capped gold nanoparticlesFacile ColorimetricHuman SerumsensorRSDfactors pHcolor conversionSpectrophotometric Methodnaked-eye color changeCitrate-Capped Gold Nanoparticles
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