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A Dual-Signal Twinkling Probe for Fluorescence-SERS Dual Spectrum Imaging and Detection of miRNA in Single Living Cell via Absolute Value Coupling of Reciprocal Signals
journal contribution
posted on 2019-03-29, 00:00 authored by Na Zhang, Sujuan Ye, Zhenxing Wang, Ronghua Li, Menglei WangImaging and detecting
microRNAs (miRNAs) is of central importance
in tumor cell analysis. It stays challenging to establish simple,
accurate, and sensitive analytical assays for imaging and detection
of miRNA in a single living cell, because of intracellular complex
environment and miRNA sequence similarity. Herein, we designed a dual-signal
twinkling probe (DSTP) with triplex-stem structure which employed
a fluorescence-SERS signal reciprocal switch. The spatiotemporal dynamics
of the miRNA molecular and intracellular uptake of the probe are monitored
by fluorescence-SERS signal switch of the DSTP. Meanwhile, using the
surface-enhanced Raman scattering (SERS) signals of DSTP, the measure
of absolute value coupling of reciprocal signals is first used to
real-time detection of miRNA. Through simultaneous enhancing the target
response signal value and reducing blank value, this work deducted
the background effect, and showed high sensitivity and reproducibility.
Moreover, the probe shows excellent reversibility and specificity
in real quantitative detection of intracellular miRNA. miR-203 was
successfully monitored in MCF-7, in accord with the results in vitro
as well as in cell lysates. We anticipate that this new dual-signal
twinkling and dual-spectrum switch method will be generally useful
to image and detect various types of biomolecules in single living
cell.
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Keywords
Reciprocal Signals ImagingSpectrum Imagingprobesurface-enhanced Ramanintracellular miRNAdual-spectrum switch methodSingle Living CellAbsolute Valuefluorescence-SERS signalmiRNA sequence similaritybackground effectmiR -203cell lysatesDSTPtarget response signal valuespatiotemporal dynamicstriplex-stem structuretumor cell analysisdetectionDual-Signal Twinkling ProbeMCFintracellular uptakefluorescence-SERS signal switch
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