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ATR-FTIR Spectroscopy Supported by Multivariate Analysis for the Characterization of Adipose Tissue Aspirates from Patients Affected by Systemic Amyloidosis
journal contribution
posted on 2019-01-24, 00:00 authored by Diletta Ami, Paolo Mereghetti, Andrea Foli, Masayoshi Tasaki, Paolo Milani, Mario Nuvolone, Giovanni Palladini, Giampaolo Merlini, Francesca Lavatelli, Antonino NatalelloDeposition
of misfolded proteins as extracellular amyloid aggregates
is the pathological hallmark of systemic amyloidoses. Subcutaneous
fat acquired by fine needle aspiration is the preferred screening
tissue in suspected patients. In this study we employed Fourier transform
infrared (FTIR) spectroscopy in attenuated total reflection (ATR)
to investigate human abdominal fat aspirates with the aim of detecting
disease-related changes in the molecular structure and composition
of the tissue and exploiting the potentiality of the method to discriminate
between amyloid-positive and -negative samples. The absorption and
second-derivative spectra of Congo Red (CR) positive and CR-negative
specimens were analyzed by three multivariate methods in four spectral
regions. The proposed ATR-FTIR method is label-free, rapid, and relatively
inexpensive and requires minimal sample preparation. We found that
the ATR-FTIR approach can differentiate fat aspirates containing amyloid
deposits from control specimens with high sensitivity and specificity,
both at 100 [89–100]%. It is worth noting that the wavenumbers
most important for discrimination indicate that changes both in the
protein conformation and in resident lipids are intrinsic features
of affected subcutaneous fat in comparison with the CR-negative controls.
In this proof of concept study, we show that this approach could be
useful for assessing tissue amyloid aggregates and for acquiring novel
knowledge of the molecular bases of the disease.
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Keywords
Multivariate AnalysisSystemic Amyloidosis DepositionCongo Redtissue amyloid aggregatesmultivariate methodsnovel knowledgesecond-derivative spectrasample preparationextracellular amyloid aggregatesprotein conformationCR-negative specimensdisease-related changesneedle aspirationATR-FTIR methodconcept studyamyloid depositsATR-FTIR approachcontrol specimensATR-FTIR Spectroscopyscreening tissueAdipose Tissue Aspiratesmisfolded proteinsCR-negative controlsaspirateresident lipids
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