Antimicrobial Peptide-Conjugated Hierarchical Antifouling
Polymer Brushes for Functionalized Catheter Surfaces
Xin-Yang Zhang
Yu-Qing Zhao
Yidan Zhang
Anzhi Wang
Xiaokang Ding
Yang Li
Shun Duan
Xuejia Ding
Fu-Jian Xu
10.1021/acs.biomac.9b01060.s001
https://acs.figshare.com/articles/journal_contribution/Antimicrobial_Peptide-Conjugated_Hierarchical_Antifouling_Polymer_Brushes_for_Functionalized_Catheter_Surfaces/9996539
Catheter-related
infection is a great challenge to modern medicine,
which causes significant economic burden and increases patient morbidity.
Hence, there is a great requirement for functionalized surfaces with
inherently antibacterial properties and biocompatibility that prevent
bacterial colonization and attachment of blood cells. Herein, we developed
a strategy for constructing polymer brushes with hierarchical architecture
on polyurethane (PU) via surface-initiated atom-transfer radical polymerization
(SI-ATRP). Surface-functionalized PU (PU-DMH) was readily prepared,
which comprised of poly(3-[dimethyl-[2-(2-methylprop-2-enoyloxy)ethyl]azaniumyl]propane-1-sulfonate)
(PDMAPS) brushes as the lower layer and antimicrobial peptide-conjugated
poly(methacrylic acid) (PMAA) brushes as the upper layer. The PU-DMH
surface showed excellent bactericidal property against both Gram-positive
and Gram-negative bacteria and could prevent accumulation of bacterial
debris on surfaces. Simultaneously, the PU-DMH samples possessed good
hemocompatibility and low cytotoxicity. Furthermore, the integrated
antifouling and bactericidal properties of PU-DMH under hydrodynamic
conditions were confirmed by an in vitro circulating model. The functionalized
surface possessed persistent antifouling and bactericidal performances
both under static and hydrodynamic conditions. The microbiological
and histological results of animal experiments also verified the in
vivo anti-infection performance. The present work might find promising
clinical applications for preventing catheter-related infection.
2019-10-17 17:35:02
Functionalized Catheter Surfaces Catheter-related infection
PDMAPS
antifouling
functionalized
bactericidal
PU
increases patient morbidity
Antimicrobial Peptide-Conjugated Hierarchical Antifouling Polymer Brushes
SI-ATRP
poly
PU-DMH
PMAA
surface
hydrodynamic conditions
vivo anti-infection performance