Investigating Phosphorylation-Induced Conformational Changes in WNK1 Kinase by Molecular Dynamics Simulations Nisha Amarnath Jonniya Md Fulbabu Sk Parimal Kar 10.1021/acsomega.9b02187.s001 https://acs.figshare.com/articles/journal_contribution/Investigating_Phosphorylation-Induced_Conformational_Changes_in_WNK1_Kinase_by_Molecular_Dynamics_Simulations/9970190 The With-No-Lysine (WNK) kinase is considered to be a master regulator for various cation-chloride cotransporters involved in maintaining cell-volume and ion homeostasis. Here, we have investigated the phosphorylation-induced structural dynamics of the WNK1 kinase bound to an inhibitor via atomistic molecular dynamics simulations. Results from our simulations show that the phosphorylation at Ser<sup>382</sup> could stabilize the otherwise flexible activation loop (A-loop). The intrahelix salt-bridge formed between Arg<sup>264</sup> and Glu<sup>268</sup> in the unphosphorylated system is disengaged after the phosphorylation, and Glu<sup>268</sup> reorients itself and forms a stable salt-bridge with Arg<sup>348</sup>. The dynamic cross-correlation analysis shows that phosphorylation diminishes anticorrelated motions and increases correlated motions between different domains. Structural network analysis reveals that the phosphorylation causes structural rearrangements and shortens the communication path between the αC-helix and catalytic loop, making the binding pocket more suitable for accommodating the ligand. Overall, we have characterized the structural changes in the WNK kinase because of phosphorylation in the A-loop, which might help in designing rational drugs. 2019-10-11 09:03:20 Phosphorylation-Induced Conformational Changes Arg 348 Arg 264 cross-correlation analysis activation loop Glu 268 reorients α C-helix Molecular Dynamics Simulations Glu 268 WNK 1 Kinase simulations show WNK kinase Ser 382 A-loop Structural network analysis phosphorylation causes binding pocket anticorrelated motions unphosphorylated system master regulator communication path cation-chloride cotransporters ion homeostasis dynamics simulations intrahelix salt-bridge WNK 1 kinase