10.1021/acs.jproteome.9b00429.s002
Wenjing Peng
Wenjing
Peng
Mona Goli
Mona
Goli
Parvin Mirzaei
Parvin
Mirzaei
Yehia Mechref
Yehia
Mechref
Revealing the
Biological Attributes of N‑Glycan
Isomers in Breast Cancer Brain Metastasis Using Porous Graphitic Carbon
(PGC) Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)
American Chemical Society
2019
breast cancer brain metastasis
role glycan isomers
231 BR
50 N-glycan compositions
expression alterations
Breast Cancer Brain Metastasis
Liquid Chromatography-Tandem Mass Spectrometry
breast cancer cells
sialylated glycan isomers
N-glycan isomer function
glycan isomer expressions
breast cancer cell lines
Porous Graphitic Carbon
breast cancer invasion
brain-seeking cell line
brain cancer cell line
breast cancer patients
LC-MS
PGC
brain-seeking cell line 231 BR
breast cancer metastasis
roles glycan isomers
2019-09-16 12:36:25
Dataset
https://acs.figshare.com/articles/dataset/Revealing_the_Biological_Attributes_of_N_Glycan_Isomers_in_Breast_Cancer_Brain_Metastasis_Using_Porous_Graphitic_Carbon_PGC_Liquid_Chromatography-Tandem_Mass_Spectrometry_LC-MS_MS_/9850970
Breast cancer is
a leading cancer in women and is considered to
be the second-most common metastatic cancer following lung cancer.
An estimated 10–16% of breast cancer patients are suffering
from brain metastasis, and the diagnostic cases of breast cancer brain
metastasis are increasing. Nevertheless, the mechanisms behind this
process are still unclear. Aberrant glycosylation has been proved
to be related to many diseases and cancer metastasis. However, studies
of N-glycan isomer function in breast cancer brain metastasis are
limited. In this study, the expressions of N-glycan isomers derived
from five breast cancer cell lines and one brain cancer cell line
were investigated and compared to a brain-seeking cell line, 231BR,
to acquire a better understanding of the role glycan isomers play
in breast cancer brain metastasis. The high temperature nanoPGC-LC-MS/MS
achieved an efficient isomeric separation and permitted the identification
and quantitation of 144 isomers from 50 N-glycan compositions. There
were significant expression alterations of these glycan isomers among
the different breast cancer cell lines. The increase of total glycan
abundance and sialylation level were observed to be associated with
breast cancer invasion. With regard to individual isomers, the greatest
number of sialylated isomers was observed along with significant expression
alterations in 231BR, suggesting a relationship between glycan sialylation
and breast cancer brain metastasis. Furthermore, the increase of the
α2,6-sialylation level in 231BR likely contributes to the passage
of breast cancer cells through the blood-brain barrier, thus facilitating
breast cancer brain metastasis. Meanwhile, the upregulation of highly
sialylated glycan isomers with α2,6-linked sialic acids were
found to be associated with breast cancer metastasis. This investigation
of glycan isomer expressions, especially the unique isomeric expression
in brain-seeking cell line 231BR, provides new information toward
understanding the potential roles glycan isomers play during breast
cancer metastasis and more clues for a deeper insight of this bioprocess.