Enhanced Chemical Stability, Intestinal Absorption,
and Intracellular Antioxidant Activity of Cyanidin-3‑<i>O</i>‑glucoside by Composite Nanogel Encapsulation
Jin Feng
Yinghui Wu
Lixia Zhang
Ying Li
Songbai Liu
Hua Wang
Chunyang Li
10.1021/acs.jafc.9b04778.s001
https://acs.figshare.com/articles/journal_contribution/Enhanced_Chemical_Stability_Intestinal_Absorption_and_Intracellular_Antioxidant_Activity_of_Cyanidin-3_i_O_i_glucoside_by_Composite_Nanogel_Encapsulation/9792851
A composite
nanogel was developed for cyanidin-3-<i>O</i>-glucoside
(C3G) delivery by combining Maillard reaction and heat
gelation. The starting materials utilized were ovalbumin, dextran,
and pectin. C3G-loaded nanogel was spherical with a diameter of ∼185
nm, which was maintained over a wide range of pH and NaCl concentrations.
The composite nanogel enhanced the chemical stability of C3G under
accelerated degradation models and a simulated gastrointestinal tract.
Clathrin-mediated, caveolae-mediated, and macropinocytosis-related
endocytosis contributed to the higher cellular uptake of nano-C3G
than that of free-C3G. The apparent permeability coefficients of C3G
increased 2.16 times after nanoencapsulation. The transcytosis of
the C3G-bearing nanogel occurred primarily through the clathrin-related
pathway and macropinocytosis and followed the “common recycling
endosomes–endoplasmic reticulum–Golgi complex–basolateral
plasma membrane” route. Moreover, nano-C3G was more efficient
in restoring the viability of cells and activities of endogenous antioxidant
enzymes than free-C3G in oxidative models, which may be attributed
to the former’s high cellular absorption.
2019-09-10 14:58:38
Intracellular Antioxidant Activity
cyanidin -3- O
free-C 3G
C 3G
C 3G delivery
Composite Nanogel Encapsulation
Enhanced Chemical Stability
C 3G nanogel
nano-C 3G