10.1021/acs.jafc.9b04778.s001
Jin Feng
Jin
Feng
Yinghui Wu
Yinghui
Wu
Lixia Zhang
Lixia
Zhang
Ying Li
Ying
Li
Songbai Liu
Songbai
Liu
Hua Wang
Hua
Wang
Chunyang Li
Chunyang
Li
Enhanced Chemical Stability, Intestinal Absorption,
and Intracellular Antioxidant Activity of Cyanidin-3‑<i>O</i>‑glucoside by Composite Nanogel Encapsulation
American Chemical Society
2019
Intracellular Antioxidant Activity
cyanidin -3- O
free-C 3G
C 3G
C 3G delivery
Composite Nanogel Encapsulation
Enhanced Chemical Stability
C 3G nanogel
nano-C 3G
2019-09-10 14:58:38
Journal contribution
https://acs.figshare.com/articles/journal_contribution/Enhanced_Chemical_Stability_Intestinal_Absorption_and_Intracellular_Antioxidant_Activity_of_Cyanidin-3_i_O_i_glucoside_by_Composite_Nanogel_Encapsulation/9792851
A composite
nanogel was developed for cyanidin-3-<i>O</i>-glucoside
(C3G) delivery by combining Maillard reaction and heat
gelation. The starting materials utilized were ovalbumin, dextran,
and pectin. C3G-loaded nanogel was spherical with a diameter of ∼185
nm, which was maintained over a wide range of pH and NaCl concentrations.
The composite nanogel enhanced the chemical stability of C3G under
accelerated degradation models and a simulated gastrointestinal tract.
Clathrin-mediated, caveolae-mediated, and macropinocytosis-related
endocytosis contributed to the higher cellular uptake of nano-C3G
than that of free-C3G. The apparent permeability coefficients of C3G
increased 2.16 times after nanoencapsulation. The transcytosis of
the C3G-bearing nanogel occurred primarily through the clathrin-related
pathway and macropinocytosis and followed the “common recycling
endosomes–endoplasmic reticulum–Golgi complex–basolateral
plasma membrane” route. Moreover, nano-C3G was more efficient
in restoring the viability of cells and activities of endogenous antioxidant
enzymes than free-C3G in oxidative models, which may be attributed
to the former’s high cellular absorption.