Production of Amphidinols and Other Bioproducts of
Interest by the Marine Microalga Amphidinium carterae Unraveled by Nuclear Magnetic Resonance Metabolomics Approach Coupled
to Multivariate Data Analysis
Ana Cristina Abreu
Alejandro Molina-Miras
Luis M. Aguilera-Sáez
Lorenzo López-Rosales
María del Carmen Cerón-García
Asterio Sánchez-Mirón
Lucía Olmo-García
Alegría Carrasco-Pancorbo
Francisco García-Camacho
Emilio Molina-Grima
Ignacio Fernández
10.1021/acs.jafc.9b02821.s001
https://acs.figshare.com/articles/journal_contribution/Production_of_Amphidinols_and_Other_Bioproducts_of_Interest_by_the_Marine_Microalga_Amphidinium_carterae_Unraveled_by_Nuclear_Magnetic_Resonance_Metabolomics_Approach_Coupled_to_Multivariate_Data_Analysis/9636644
This study assessed
the feasibility of an NMR metabolomics approach
coupled to multivariate data analysis to monitor the naturally present
or stresses-elicited metabolites from a long-term (>170 days) culture
of the dinoflagellate marine microalgae Amphidinium
carterae grown in a fiberglass paddlewheel-driven
raceway photobioreactor. Metabolic contents, in particular, in two
members of the amphidinol family, amphidinol A and its 7-sulfate derivative
amphidinol B (referred as APDs), and other compounds of interest (fatty
acids, carotenoids, oxylipins, etc.) were evaluated by altering concentration
levels of the f/2 medium nutrients and daily mean irradiance. Operating
with a 24 h sinusoidal light cycle allowed a 3-fold increase in APD
production, which was also detected by an increase in hemolytic activity
of the methanolic extract of A. carterae biomass. The presence of APDs was consistent with the antitumoral
activity measured in the methanolic extracts of the biomass. Increased
daily irradiance was accompanied by a general decrease in pigments
and an increase in SFAs (saturated fatty acids), MUFAs (monounsaturated
fatty acids), and DHA (docosahexaenoic acid), while increased nutrient
availability lead to an increase in sugar, amino acid, and PUFA ω-3
contents and pigments and a decrease in SFAs and MUFAs. NMR-based
metabolomics is shown to be a fast and suitable method to accompany
the production of APD and bioactive compounds without the need of
tedious isolation methods and bioassays. The two APD compounds were
chemically identified by spectroscopic NMR and spectrometric ESI-IT
MS (electrospray ionization ion trap mass spectrometry) and ESI-TOF
MS (ESI time-of-flight mass spectrometry) methods.
2019-08-15 18:06:58
irradiance
ESI-IT MS
carterae biomass
APD compounds
Marine Microalga Amphidinium carterae Unraveled
pigment
stresses-elicited metabolites
concentration levels
light cycle
dinoflagellate marine microalgae Amphidinium carterae
acid
amphidinol family
PUFA ω-3 contents
ESI time-of-flight mass spectrometry
Other Bioproducts
SFA
antitumoral activity
isolation methods
methanolic extracts
spectroscopic NMR
7- sulfate
ESI-TOF MS
MUFA
multivariate data analysis
NMR metabolomics approach
electrospray ionization ion trap mass spectrometry
fiberglass paddlewheel-driven raceway photobioreactor
bioactive compounds
24 h
NMR-based metabolomics
DHA
amphidinol B
Resonance Metabolomics Approach
APD production
Multivariate Data Analysis
Metabolic contents