10.1021/acsnano.9b02291.s002
Hyungjun Kim
Hyungjun
Kim
Min Ku Kim
Min Ku
Kim
Hanmin Jang
Hanmin
Jang
Bongjoong Kim
Bongjoong
Kim
Dong Rip Kim
Dong Rip
Kim
Chi Hwan Lee
Chi Hwan
Lee
Sensor-Instrumented
Scaffold Integrated with Microporous Spongelike Ultrabuoy for Long-Term
3D Mapping of Cellular Behaviors
and Functions
American Chemical Society
2019
Long-Term 3 D Mapping
Functions Real-time monitoring
ultrabuoyant 3 D
sensor-instrumented scaffolds
cell culture conditions
Microporous Spongelike Ultrabuoy
Sensor-Instrumented Scaffold Integrated
instrument settings
disease modeling
electrophysiological signals
function
Cellular Behaviors
vivo tests
tissue development
drug screening
high-fidelity recording
culture medium
2019-06-19 00:00:00
Media
https://acs.figshare.com/articles/media/Sensor-Instrumented_Scaffold_Integrated_with_Microporous_Spongelike_Ultrabuoy_for_Long-Term_3D_Mapping_of_Cellular_Behaviors_and_Functions/8311775
Real-time
monitoring of cellular behaviors and functions with sensor-instrumented
scaffolds can provide a profound impact on fundamental studies of
the underlying biophysics and disease modeling. Although quantitative
measurement of predictive data for <i>in vivo</i> tests
and physiologically relevant information in these contexts is important,
the long-term reliable monitoring of cellular functions in three-dimensional
(3D) environments is limited by the required set under wet cell culture
conditions that are unfavorable to electronic instrument settings.
Here, we introduce an ultrabuoyant 3D instrumented scaffold that can
remain afloat on the surface of culture medium and thereby provides
favorable environments for the entire electronic components in the
air while the cells reside and grow underneath. This setting enables
high-fidelity recording of electrical cell–substrate impedance
and electrophysiological signals for a long period of time (weeks).
Comprehensive <i>in vitro</i> studies reveal the utility
of this platform as an effective tool for drug screening and tissue
development.