%0 Online Multimedia
%A Kim, Hyungjun
%A Kim, Min Ku
%A Jang, Hanmin
%A Kim, Bongjoong
%A Kim, Dong Rip
%A Lee, Chi Hwan
%D 2019
%T Sensor-Instrumented
Scaffold Integrated with Microporous Spongelike Ultrabuoy for Long-Term
3D Mapping of Cellular Behaviors
and Functions
%U https://acs.figshare.com/articles/media/Sensor-Instrumented_Scaffold_Integrated_with_Microporous_Spongelike_Ultrabuoy_for_Long-Term_3D_Mapping_of_Cellular_Behaviors_and_Functions/8311772
%R 10.1021/acsnano.9b02291.s001
%2 https://acs.figshare.com/ndownloader/files/15573662
%K Long-Term 3 D Mapping
%K Functions Real-time monitoring
%K ultrabuoyant 3 D
%K sensor-instrumented scaffolds
%K cell culture conditions
%K Microporous Spongelike Ultrabuoy
%K Sensor-Instrumented Scaffold Integrated
%K instrument settings
%K disease modeling
%K electrophysiological signals
%K function
%K Cellular Behaviors
%K vivo tests
%K tissue development
%K drug screening
%K high-fidelity recording
%K culture medium
%X Real-time
monitoring of cellular behaviors and functions with sensor-instrumented
scaffolds can provide a profound impact on fundamental studies of
the underlying biophysics and disease modeling. Although quantitative
measurement of predictive data for in vivo tests
and physiologically relevant information in these contexts is important,
the long-term reliable monitoring of cellular functions in three-dimensional
(3D) environments is limited by the required set under wet cell culture
conditions that are unfavorable to electronic instrument settings.
Here, we introduce an ultrabuoyant 3D instrumented scaffold that can
remain afloat on the surface of culture medium and thereby provides
favorable environments for the entire electronic components in the
air while the cells reside and grow underneath. This setting enables
high-fidelity recording of electrical cell–substrate impedance
and electrophysiological signals for a long period of time (weeks).
Comprehensive in vitro studies reveal the utility
of this platform as an effective tool for drug screening and tissue
development.
%I ACS Publications