Sensor-Instrumented Scaffold Integrated with Microporous Spongelike Ultrabuoy for Long-Term 3D Mapping of Cellular Behaviors and Functions Hyungjun Kim Min Ku Kim Hanmin Jang Bongjoong Kim Dong Rip Kim Chi Hwan Lee 10.1021/acsnano.9b02291.s001 https://acs.figshare.com/articles/media/Sensor-Instrumented_Scaffold_Integrated_with_Microporous_Spongelike_Ultrabuoy_for_Long-Term_3D_Mapping_of_Cellular_Behaviors_and_Functions/8311772 Real-time monitoring of cellular behaviors and functions with sensor-instrumented scaffolds can provide a profound impact on fundamental studies of the underlying biophysics and disease modeling. Although quantitative measurement of predictive data for <i>in vivo</i> tests and physiologically relevant information in these contexts is important, the long-term reliable monitoring of cellular functions in three-dimensional (3D) environments is limited by the required set under wet cell culture conditions that are unfavorable to electronic instrument settings. Here, we introduce an ultrabuoyant 3D instrumented scaffold that can remain afloat on the surface of culture medium and thereby provides favorable environments for the entire electronic components in the air while the cells reside and grow underneath. This setting enables high-fidelity recording of electrical cell–substrate impedance and electrophysiological signals for a long period of time (weeks). Comprehensive <i>in vitro</i> studies reveal the utility of this platform as an effective tool for drug screening and tissue development. 2019-06-19 00:00:00 Long-Term 3 D Mapping Functions Real-time monitoring ultrabuoyant 3 D sensor-instrumented scaffolds cell culture conditions Microporous Spongelike Ultrabuoy Sensor-Instrumented Scaffold Integrated instrument settings disease modeling electrophysiological signals function Cellular Behaviors vivo tests tissue development drug screening high-fidelity recording culture medium