10.1021/acs.molpharmaceut.8b01274.s001
Jason Kuhn
Jason
Kuhn
Asya Smirnov
Asya
Smirnov
Alison K. Criss
Alison K.
Criss
Linda Columbus
Linda
Columbus
Quantifying Carcinoembryonic Antigen-like Cell Adhesion
Molecule-Targeted Liposome Delivery Using Imaging Flow Cytometry
American Chemical Society
2019
CEACAM 1-mediated uptake
lysosomal trafficking compartments
imaging flow cytometry
liposomes
membrane protein Opa
Quantifying Carcinoembryonic Antigen-like Cell Adhesion Molecule-Targeted Liposome Delivery
HeLa cells
CEACAM 1
Imaging Flow Cytometry Carcinoembryonic antigen-like cell adhesion molecules
2019-04-17 00:00:00
Journal contribution
https://acs.figshare.com/articles/journal_contribution/Quantifying_Carcinoembryonic_Antigen-like_Cell_Adhesion_Molecule-Targeted_Liposome_Delivery_Using_Imaging_Flow_Cytometry/8118464
Carcinoembryonic
antigen-like cell adhesion molecules (CEACAMs)
are human cell-surface proteins that can exhibit increased expression
on tumor cells and are thus a potential target for novel tumor-seeking
therapeutic delivery methods. We hypothesize that engineered nanoparticles
containing a known interaction partner of CEACAM, Neisseria
gonorrhoeae outer membrane protein Opa, can be used
to deliver cargo to specific cellular targets. In this study, the
cell association and uptake of protein-free liposomes and Opa proteoliposomes
into CEACAM-expressing cells were measured using imaging flow cytometry.
A size-dependent internalization of liposomes into HeLa cells was
observed through endocytic pathways. Opa-dependent, CEACAM1-mediated
uptake of liposomes into HeLa cells was observed, with limited colocalization
with endosomal and lysosomal trafficking compartments. Given the overexpression
of CEACAM1 on several distinct cancers and interest in using CEACAM1
as a component in treatment strategies, these results support further
pursuit of investigating Opa-dependent specificity and the internalization
mechanism for therapeutic delivery.