Dissecting the Components of Sindbis Virus from Arthropod
and Vertebrate Hosts: Implications for Infectivity Differences
Carmen A. Dunbar
Vamseedhar Rayaprolu
Joseph C.-Y. Wang
Christopher J. Brown
Emma Leishman
Sara Jones-Burrage
Jonathan C. Trinidad
Heather B. Bradshaw
David E. Clemmer
Suchetana Mukhopadhyay
Martin F. Jarrold
10.1021/acsinfecdis.8b00356.s001
https://acs.figshare.com/articles/journal_contribution/Dissecting_the_Components_of_Sindbis_Virus_from_Arthropod_and_Vertebrate_Hosts_Implications_for_Infectivity_Differences/7996091
Sindbis virus (SINV) is an enveloped,
single-stranded RNA virus, which is transmitted via mosquitos to a
wide range of vertebrate hosts. SINV produced by vertebrate, baby
hamster kidney (BHK) cells is more than an order of magnitude less
infectious than SINV produced from mosquito (C6/36) cells. The cause
of this difference is poorly understood. In this study, charge detection
mass spectrometry was used to determine the masses of intact SINV
particles isolated from BHK and C6/36 cells. The measured masses are
substantially different: 52.88 MDa for BHK derived SINV and 50.69
MDa for C6/36 derived. Further analysis using several mass spectrometry-based
methods and biophysical approaches indicates that BHK derived SINV
has a substantially higher mass than C6/36 derived because in the
lipid bilayer, there is a higher portion of lipids containing long
chain fatty acids. The difference in lipid composition could influence
the organization of the lipid bilayer. As a result, multiple stages
of the viral lifecycle may be affected including assembly and budding,
particle stability during transmission, and fusion events, all of
which could contribute to the differences in infectivity.
2019-04-15 19:36:33
SINV
Infectivity Differences Sindbis virus
baby hamster kidney
single-stranded RNA virus
charge detection mass spectrometry
lipid bilayer
mass spectrometry-based methods
BHK