%0 Journal Article %A Sharifian Gh., Mohammad %A Wilhelm, Michael J. %A Moore, Michael %A Dai, Hai-Lung %D 2019 %T Spatially Resolved Membrane Transport in a Single Cell Imaged by Second Harmonic Light Scattering %U https://acs.figshare.com/articles/journal_contribution/Spatially_Resolved_Membrane_Transport_in_a_Single_Cell_Imaged_by_Second_Harmonic_Light_Scattering/7914251 %R 10.1021/acs.biochem.9b00110.s001 %2 https://acs.figshare.com/ndownloader/files/14737772 %K transport rates %K time-resolved SH images %K Single Cell Imaged %K membrane %K Second Harmonic Light Scattering %X We demonstrate that time-resolved second harmonic (SH) light scattering, when applied as an imaging modality, can be used to spatially resolve the adsorption and transport rates of molecules diffusing across the membrane in a living cell. As a representative example, we measure the passive transport of the amphiphilic ion, malachite green, across the plasma membrane in living human dermal fibroblast cells. Analysis of the time-resolved SH images reveals that membrane regions, which appear to be enduring higher stress, exhibit slower transport rates. It is proposed that this stress-transport relation may be a result of local enrichment of membrane rigidifiers as part of a response to maintain membrane integrity under strain. %I ACS Publications