One Approach for Two: Toward the Creation of Near-Infrared
Imaging Agents and Rapid Screening of Lanthanide(III) Ion Sensitizers
Using Polystyrene Nanobeads
Ivana Martinić
Svetlana V. Eliseeva
Guillaume Collet
Tian-Yi Luo
Nathaniel Rosi
Stéphane Petoud
10.1021/acsabm.9b00053.s001
https://acs.figshare.com/articles/journal_contribution/One_Approach_for_Two_Toward_the_Creation_of_Near-Infrared_Imaging_Agents_and_Rapid_Screening_of_Lanthanide_III_Ion_Sensitizers_Using_Polystyrene_Nanobeads/7855055
The unique luminescence properties
of lanthanide(III) ions (Ln<sup>3+</sup>) in the near-infrared (NIR) range are attracting major attention in view of their
exciting applications in the fields of technology, telecommunications,
biology, and medicine. One of the main strategies to design luminescent
Ln<sup>3+</sup>-based compounds relies on their sensitization through
appropriate chromophoric ligands. The choice of the chromophores depends
on the nature of Ln<sup>3+</sup> and, as of today, still partially
requires experimental trials, in particular, for the creation of luminescent
compounds incorporating NIR-emitting Ln<sup>3+</sup>. The synthesis
of organic ligands bearing suitable coordinating and chromophoric
units is time- and effort-consuming. We have established a strategy
to encapsulate a large number of Yb<sup>3+</sup> trifluoromethanesulfonates
and 1,<i>n</i>-dihydroxyanthraquinone chromophores in NH<sub>2</sub>-functionalized 100 nm polystyrene (PS) beads through a rapid
swelling process, leading to AQ1,<i>n</i>OH-Yb@PS/NH<sub>2</sub> (<i>n</i> = 4, 8). We have shown that 1,<i>n</i>-dihydroxyanthraquinones can act as antennae and sensitize
the characteristic Yb<sup>3+</sup> emission in the NIR upon excitation
at 465 nm. To validate the bioapplicability of these luminescent beads,
polyethylene glycol (PEG)-coated beads have been prepared (AQ1,<i>n</i>OH-Yb@PS/PEG) and demonstrated to be noncytotoxic for HeLa
cells up to 500 μg/mL. Confocal microscopy experiments have
shown that AQ1,<i>n</i>OH-Yb@PS/PEG is taken up by HeLa
cells, whereas epifluorescence microscopy confirmed the possibility
of detecting the NIR Yb<sup>3+</sup> emission in living cells. The
developed strategy has a high potential and can be further applied
for the rapid screening of sensitizers for different NIR-emitting
Ln<sup>3+</sup> ions and for the creation of smart NIR-emitting imaging
agents.
2019-03-16 19:45:05
functionalized 100 nm polystyrene
ligand
chromophore
compound
beads
creation
microscopy
dihydroxyanthraquinone
Near-Infrared Imaging Agents
PS
chromophoric
HeLa cells
NIR-emitting imaging agents
PEG
NH
strategy
emission
OH-Yb
Ln
Yb
AQ