Coleman, Christina M. Auker, Kimberly M. Killday, K. Brian Azadi, Parastoo Black, Ian Ferreira, Daneel Arabinoxyloglucan Oligosaccharides May Contribute to the Antiadhesive Properties of Porcine Urine after Cranberry Consumption Cranberry (<i>Vaccinium macrocarpon</i>) juice is traditionally used for the prevention of urinary tract infections. Human urine produced after cranberry juice consumption can prevent <i>Escherichia coli</i> adhesion, but the antiadhesive urinary metabolites responsible have not been conclusively identified. Adult female sows were therefore fed spray-dried cranberry powder (5 g/kg/day), and urine was collected via catheter. Urine fractions were tested for antiadhesion activity using a human red blood cell (A+) anti-hemagglutination assay with uropathogenic P-fimbriated <i>E. coli</i>. Components were isolated from fractions of interest using Sephadex LH-20 gel filtration chromatography followed by HPLC on normal and reversed-phase sorbents with evaporative light scattering detection. Active urine fractions were found to contain a complex series of oligosaccharides but not proanthocyanidins, and a single representative arabinoxyloglucan octasaccharide was isolated in sufficient quantity and purity for full structural characterization by chemical derivatization and NMR spectroscopic methods. Analogous cranberry material contained a similar complex series of arabinoxyloglucan oligosaccharides that exhibited antiadhesion properties in preliminary testing. These results indicate that oligosaccharides structurally related to those found in cranberry may contribute to the antiadhesion properties of urine after cranberry consumption. Analogous cranberry material;cranberry juice consumption;uropathogenic P-fimbriated E;HPLC;oligosaccharide;detection . Active urine fractions;series;Sephadex LH -20 gel filtration chromatography;Escherichia coli adhesion;antiadhesion properties;characterization  by chemical derivatization;Urine;representative arabinoxyloglucan octasaccharide;Arabinoxyloglucan Oligosaccharides May;NMR spectroscopic methods;Cranberry Consumption Cranberry 2019-03-15
    https://acs.figshare.com/articles/journal_contribution/Arabinoxyloglucan_Oligosaccharides_May_Contribute_to_the_Antiadhesive_Properties_of_Porcine_Urine_after_Cranberry_Consumption/7851059
10.1021/acs.jnatprod.8b01043.s002