10.1021/acs.langmuir.9b00194.s001
Xiaoting Fu
Xiaoting
Fu
Seiichi Ohta
Seiichi
Ohta
Masamichi Kamihira
Masamichi
Kamihira
Yasuyuki Sakai
Yasuyuki
Sakai
Taichi Ito
Taichi
Ito
Size-Controlled Preparation of Microsized Perfluorocarbon
Emulsions as Oxygen Carriers via the Shirasu Porous Glass Membrane
Emulsification Technique
American Chemical Society
2019
oxygen concentration
OC
autoclave sterilization
Oxygen Carriers
show fluorescence
Size-Controlled Preparation
blood cells
size distribution
HeLa cells
oxygen carriers
glass membrane emulsification
Microsized Perfluorocarbon Emulsions
microsized perfluorocarbon
2 D
Monodispersed PFC emulsions
Shirasu Porous Glass Membrane Emulsification Technique
F 127-stabilized microsized PFC emulsions
3 D
membrane pore size
2019-02-21 00:00:00
Journal contribution
https://acs.figshare.com/articles/journal_contribution/Size-Controlled_Preparation_of_Microsized_Perfluorocarbon_Emulsions_as_Oxygen_Carriers_via_the_Shirasu_Porous_Glass_Membrane_Emulsification_Technique/7804607
We have developed
microsized perfluorocarbon (PFC) emulsions with
different sizes as artificial oxygen carriers (OCs) via Shirasu porous
glass membrane emulsification. Monodispersed PFC emulsions with narrow
size distribution were obtained. By changing the membrane pore size,
we were able to precisely control the size of emulsions and fabricate
emulsions similar in size to human red blood cells. Behaviors of Pluronics
with different physiochemical properties (F127, F68, P85, and P103)
as surfactants were also investigated, which evidenced that the type
and concentration of Pluronics have a major impact on the size of
emulsions and the response to different thermal conditions. The F127-stabilized
microsized PFC emulsions were stable even during autoclave sterilization.
The emulsions were loaded with Ru(ddp)an oxygen-sensitive
probeon their surfaces to indicate oxygen concentration. Finally,
incubations with HeLa cells that show fluorescence in response to
hypoxia cultured in 2D and 3D suggested promising potential of our
emulsions for OCs.