10.1021/acsabm.9b00023.s001
Claudia Tallian
Claudia
Tallian
Gregor Tegl
Gregor
Tegl
Lisa Quadlbauer
Lisa
Quadlbauer
Robert Vielnascher
Robert
Vielnascher
Simone Weinberger
Simone
Weinberger
Raymon Cremers
Raymon
Cremers
Alessandro Pellis
Alessandro
Pellis
Johannes W.O. Salari
Johannes W.O.
Salari
Georg M. Guebitz
Georg M.
Guebitz
Lysozyme-Responsive Spray-Dried Chitosan Particles
for Early Detection of Wound Infection
American Chemical Society
2019
Lysozyme-Responsive Spray-Dried Chitosan Particles
wound infections
acetylated chitosan particles
acetylated chitosan flakes
Wound Infection Infections
confocal laser scanning microscopy
scanning electron microscopy
surface
infection detection system
2019-02-19 00:00:00
Journal contribution
https://acs.figshare.com/articles/journal_contribution/Lysozyme-Responsive_Spray-Dried_Chitosan_Particles_for_Early_Detection_of_Wound_Infection/7780895
Infections are a
severe health issue, and the need for an early
point-of-care diagnostic approach for wound infections is continuously
growing. Lysozyme has shown a great potential as a biomarker for rapid
detection of wound infection. In this study, spray-drying of labeled
and derivatized chitosans was investigated for the production of small
particles responsive to lysozyme. Therefore, various chitosans, differing
in their origin (snow crab, <i>Chionoecetes sp.</i>, with
medium and low molecular weight or shrimp) were <i>N</i>-acetylated, labeled with reactive black 5, and tested for solubility
and spray-drying suitability. Reactive black-5-stained <i>N</i>-acetylated chitosan (low molecular weight, origin crab) was successfully
spray-dried, and the obtained particles were characterized regarding
size, ζ potential, and morphology. The particles showed an average
hydrodynamic radius of 612.5 ± 132.8 nm. ζ potential was
measured in the context of a later application as an infection detection
system for wound infections in artificial wound fluid (−6.14
± 0.16 mV) and infected wound fluid (−7.93 ± 1.35
mV). Furthermore, the aggregation behavior and surface structure were
analyzed by using scanning electron microscopy and confocal laser
scanning microscopy revealing spherical-shaped particles with explicit
surface topologies. Spray-dried <i>N</i>-acetylated chitosan
particles showed a 5-fold increase in lysozyme-responsive release
of dyed chitosan fragments due to the enhanced surface area to volume
ratio when compared to non-spray-dried <i>N</i>-acetylated
chitosan flakes. On the basis of these results, the study showed the
improved properties of <i>N</i>-acetylated spray-dried chitosan
particles for future applications for early and rapid infection detection.