Highly Efficient and Enzyme-Recoverable Method for
Enzymatic Concentrating Omega‑3 Fatty Acids Generated by Hydrolysis
of Fish Oil in a Substrate-Constituted Three-Liquid-Phase System
Zhigang Li
Hua Chen
Jinfen Su
Weifei Wang
Huayong Chen
Bo Yang
Yonghua Wang
10.1021/acs.jafc.8b06382.s001
https://acs.figshare.com/articles/journal_contribution/Highly_Efficient_and_Enzyme-Recoverable_Method_for_Enzymatic_Concentrating_Omega_3_Fatty_Acids_Generated_by_Hydrolysis_of_Fish_Oil_in_a_Substrate-Constituted_Three-Liquid-Phase_System/7749161
A novel
three-liquid-phase system which contained fish oil as the
nonpolar phase was developed for the lipase-based hydrolysis of fish
oil and subsequent enrichment of the omega-3 polyunsaturated fatty
acids (n-3 PUFA) in the glyceride fraction of the fish oil. In comparison
with the traditional oil/water system, the enrichment factor of n-3
PUFA in this system was increased by 363.4% as a result of a higher
dispersity, higher selectivity of the lipase for the other fatty acids
except for n-3PUFA, and relief of product
inhibition. The content of n-3 PUFA in the glyceride fraction could
be concentrated to 67.97% by repeated hydrolysis after removing the
free fatty acids. Furthermore, the lipase could be reused for at least
eight rounds. This method would be an ideal approach for enriching
n-3 PUFA because it is cost-effective, low in toxicity, and easily
scaled up.
2019-02-11 00:00:00
glyceride fraction
Substrate-Constituted Three-Liquid-Phase System
acid
n -3 PUFA
novel three-liquid-phase system
lipase
hydrolysis
-3PUFA
enrichment
fish oil