Selenium
Stimulates Cadmium Detoxification in <i>Caenorhabditis elegans</i> through Thiols-Mediated Nanoparticles
Formation and Secretion
Ling-Li Li
Yin-Hua Cui
Li-Ya Lu
You-Lin Liu
Chun-Jie Zhu
Li-Jiao Tian
Wen-Wei Li
Xing Zhang
Hao Cheng
Jing-Yuan Ma
Jian Chu
Zhong-Hua Tong
Han-Qing Yu
10.1021/acs.est.8b04200.s001
https://acs.figshare.com/articles/journal_contribution/Selenium_Stimulates_Cadmium_Detoxification_in_i_Caenorhabditis_elegans_i_through_Thiols-Mediated_Nanoparticles_Formation_and_Secretion/7739618
Antagonism between heavy metal and
selenium (Se) could significantly
affect their biotoxicity, but little is known about the mechanisms
underlying such microbial-mediated antagonistic processes as well
as the formed products. In this work, we examined the cadmium (Cd)–Se
interactions and their fates in <i>Caenorhabditis elegans</i> through in vivo and in vitro analysis and elucidated the machinery
of Se-stimulated Cd detoxification. Although the Se introduction induced
up to 3-fold higher bioaccumulation of Cd in <i>C. elegans</i> than the Cd-only group, the nematode viability remained at a similar
level to the Cd-only group. The relatively lower level of reactive
oxygen species in the Se & Cd group confirms a significantly enhanced
Cd detoxification by Se. The Cd–Se interaction, mediated by
multiple thiols, including glutathione and phytochelatin, resulted
in the formation of less toxic cadmium selenide (CdSe)/cadmium sulfide
(CdS) nanoparticles. The CdSe/CdS nanoparticles were mainly distributed
in the pharynx and intestine of the nematodes, and continuously excreted
from the body, which also benefitted the <i>C. elegans</i> survival. Our findings shed new light on the microbial-mediated
Cd–Se interactions and may facilitate an improved understanding
and control of Cd biotoxicity in complicated coexposure environments.
2019-02-08 00:00:00
Se-stimulated Cd detoxification
reactive oxygen species
Caenorhabditis elegans
Thiols-Mediated Nanoparticles Formation
interaction
Cd-only group