Enantioselective One-Pot Synthesis of Biaryl-Substituted Amines by Combining Palladium and Enzyme Catalysis in Deep Eutectic Solvents Juraj Paris Aline Telzerow Nicolás Ríos-Lombardía Kerstin Steiner Helmut Schwab Francisco Morís Harald Gröger Javier González-Sabín 10.1021/acssuschemeng.8b06715.s001 https://acs.figshare.com/articles/journal_contribution/Enantioselective_One-Pot_Synthesis_of_Biaryl-Substituted_Amines_by_Combining_Palladium_and_Enzyme_Catalysis_in_Deep_Eutectic_Solvents/7708055 The first application of <i>Deep Eutectic Solvents</i> (<i>DESs</i>) in asymmetric bioamination of ketones has been accomplished. The amine transaminases (ATAs) turned out to be particularly stable in <i>DES</i>-buffer mixtures at a percentage of up to 75% (w/w) neoteric solvent. Moreover, this reaction medium was used to perform a chemoenzymatic cascade toward biaryl amines by coupling a Suzuki reaction sequentially with an enantioselective bioamination catalyzed by the recently discovered ATA from <i>Exophiala xenobiotica</i> (EX-ωTA). The solubilizing properties of <i>DESs</i> enabled the metal-catalyzed step at 200 mM loading of substrate and the subsequent biotransformation at 25 mM. 2019-02-12 17:21:00 enantioselective bioamination chemoenzymatic cascade ATA DES buffer mixtures Deep Eutectic Solvents biaryl amines solubilizing properties EX -ωTA Enantioselective One-Pot Synthesis Suzuki reaction sequentially Enzyme Catalysis 200 mM loading Biaryl-Substituted Amines 25 mM reaction medium Exophiala xenobiotica amine transaminases metal-catalyzed step