10.1021/acssuschemeng.8b06715.s001 Juraj Paris Juraj Paris Aline Telzerow Aline Telzerow Nicolás Ríos-Lombardía Nicolás Ríos-Lombardía Kerstin Steiner Kerstin Steiner Helmut Schwab Helmut Schwab Francisco Morís Francisco Morís Harald Gröger Harald Gröger Javier González-Sabín Javier González-Sabín Enantioselective One-Pot Synthesis of Biaryl-Substituted Amines by Combining Palladium and Enzyme Catalysis in Deep Eutectic Solvents American Chemical Society 2019 enantioselective bioamination chemoenzymatic cascade ATA DES buffer mixtures Deep Eutectic Solvents biaryl amines solubilizing properties EX -ωTA Enantioselective One-Pot Synthesis Suzuki reaction sequentially Enzyme Catalysis 200 mM loading Biaryl-Substituted Amines 25 mM reaction medium Exophiala xenobiotica amine transaminases metal-catalyzed step 2019-02-12 17:21:00 Journal contribution https://acs.figshare.com/articles/journal_contribution/Enantioselective_One-Pot_Synthesis_of_Biaryl-Substituted_Amines_by_Combining_Palladium_and_Enzyme_Catalysis_in_Deep_Eutectic_Solvents/7708055 The first application of <i>Deep Eutectic Solvents</i> (<i>DESs</i>) in asymmetric bioamination of ketones has been accomplished. The amine transaminases (ATAs) turned out to be particularly stable in <i>DES</i>-buffer mixtures at a percentage of up to 75% (w/w) neoteric solvent. Moreover, this reaction medium was used to perform a chemoenzymatic cascade toward biaryl amines by coupling a Suzuki reaction sequentially with an enantioselective bioamination catalyzed by the recently discovered ATA from <i>Exophiala xenobiotica</i> (EX-ωTA). The solubilizing properties of <i>DESs</i> enabled the metal-catalyzed step at 200 mM loading of substrate and the subsequent biotransformation at 25 mM.