10.1021/acs.jafc.8b04944.s001
Le Chen
Le
Chen
Shigang Yao
Shigang
Yao
Tao Chen
Tao
Chen
Qin Tao
Qin
Tao
Xiangting Xie
Xiangting
Xie
Xiang Xiao
Xiang
Xiao
Derong Ding
Derong
Ding
Qin He
Qin
He
Jian He
Jian
He
Coexpression of Methyltransferase Gene <i>dmt50</i> and
Methylene Tetrahydrofolate Reductase Gene Increases <i>Arabidopsis
thaliana</i> Dicamba Resistance
American Chemical Society
2019
resistance
product 5- methyl-THF
5- methyl-THF
herbicide
dicamba-degrading strain Rhizorhabdus dicambivorans Ndbn -20. Dmt 50
mthfr
DCSA
Methyltransferase Gene dmt 50
Dmt 50 demethylation activity
dmt 50
gene
expression
field application rate
Methylene Tetrahydrofolate Reductase Gene Increases Arabidopsis thaliana Dicamba Resistance Dicamba
2019-01-17 00:00:00
Journal contribution
https://acs.figshare.com/articles/journal_contribution/Coexpression_of_Methyltransferase_Gene_i_dmt50_i_and_Methylene_Tetrahydrofolate_Reductase_Gene_Increases_i_Arabidopsis_thaliana_i_Dicamba_Resistance/7635938
Dicamba,
a broad-spectrum and highly efficient herbicide, is an
excellent target herbicide for the engineering of herbicide-resistant
crops. In this study, a new tetrahydrofolate (THF)-dependent dicamba
methyltransferase gene, <i>dmt50</i>, was cloned from the
dicamba-degrading strain <i>Rhizorhabdus dicambivorans</i> Ndbn-20. Dmt50 catalyzed the methyl transfer from dicamba to THF,
generating the herbicidally inactive product 3,6-dichlorosalicylic
acid (3,6-DCSA) and 5-methyl-THF. A <i>dmt50</i> transgenic <i>Arabidopsis thaliana</i> clearly showed dicamba resistance (560
g/ha, the normal field application rate). However, Dmt50 demethylation
activity was inhibited by the product 5-methyl-THF. Mthfr66, encoded
by the 5,10-methylene-THF reductase gene <i>mthfr66</i> could
relieve the inhibition by removing 5-methyl-THF in vitro. Compared
with expression of <i>dmt50</i> alone, simultaneous expression
of <i>dmt50</i> and <i>mthfr66</i> further improved
the dicamba resistance (1120 g/ha) of transgenic <i>A. thaliana</i>. This study provides new genes for dicamba detoxification and a
strategy for the engineering of dicamba-resistant crops.