10.1021/acsanm.8b01773.s001
Jianlei Shen
Jianlei
Shen
Yiru Zhang
Yiru
Zhang
Rong Hu
Rong
Hu
Ryan T. K. Kwok
Ryan
T. K. Kwok
Zhiming Wang
Zhiming
Wang
Anjun Qin
Anjun
Qin
Ben Zhong Tang
Ben Zhong
Tang
Dual-Mode Ultrasensitive Detection of Nucleic Acids
via an Aqueous “Seesaw” Strategy by Combining Aggregation-Induced
Emission and Plasmonic Colorimetry
American Chemical Society
2018
seesaw strategy
single-strand DNA
gold-nanoparticle-based colorimetric assay
label-free
AIE
solution tonality
acid detection strategies
acid detection strategy
enzyme-free
Aggregation-Induced Emission
Dual-Mode Ultrasensitive Detection
HCR
colorimetric assay
fluorescence intensity changes
hybridization chain reaction
37.2 fM
point-of-care testing
aggregation-induced emission
enzyme-based signal amplification
fluorometric
Nucleic Acids
pathogen diagnostics
Plasmonic Colorimetry Development
2018-11-16 00:00:00
Journal contribution
https://acs.figshare.com/articles/journal_contribution/Dual-Mode_Ultrasensitive_Detection_of_Nucleic_Acids_via_an_Aqueous_Seesaw_Strategy_by_Combining_Aggregation-Induced_Emission_and_Plasmonic_Colorimetry/7539977
Development of simple,
robust, and reliable nucleic acid detection
strategy holds tremendous promise for cancer or pathogen diagnostics.
However, in traditional fluorescent assays, tedious chemical labeling
and enzyme-based signal amplification are usually required. Developing
label-free, enzyme-free nucleic acid detection strategies is of great
significance. In this paper, through combining the hybridization chain
reaction (HCR) technique with aggregation-induced emission (AIE)-based
fluorometric assay and a gold-nanoparticle-based colorimetric assay,
we developed a “seesaw” strategy for label-free, enzyme-free
visual detection of nucleic acids. Two sets of signals (solution tonality
and fluorescence intensity changes) were generated for one test. The
limit of detection of single-strand DNA can be as low as 37.2 fM.
The proposed seesaw strategy that integrates fluorometric and colorimetric
assay holds great potential for the point-of-care testing of nucleic
acids.