10.1021/acssensors.8b01277.s002
Sune Zoëga Andreasen
Sune Zoëga
Andreasen
Kuldeep Sanger
Kuldeep
Sanger
Christian Bille Jendresen
Christian Bille
Jendresen
Alex Toftgaard Nielsen
Alex
Toftgaard Nielsen
Jenny Emnéus
Jenny
Emnéus
Anja Boisen
Anja
Boisen
Kinga Zór
Kinga
Zór
Extraction, Enrichment, and <i>in situ</i> Electrochemical Detection on Lab-on-a-Disc: Monitoring the Production
of a Bacterial Secondary Metabolite
American Chemical Society
2018
LoD
platform
data
extraction
3 μ L volume
sample
electrochemical detection
2018-12-11 00:00:00
Dataset
https://acs.figshare.com/articles/dataset/Extraction_Enrichment_and_i_in_situ_i_Electrochemical_Detection_on_Lab-on-a-Disc_Monitoring_the_Production_of_a_Bacterial_Secondary_Metabolite/7499486
Development
of microsystems, which enable “sample-to-answer”
detection from real samples, is often challenging. We present the
first integration of supported liquid membrane extraction combined
with electrochemical detection on a centrifugal fluidic platform.
The developed lab-on-a-disc (LoD) system enabled the separation, enrichment,
and subsequent electrochemical detection of the target analyte from
a complex sample mixture. As a case study, we quantified the amount
of a dietary supplement and pharmaceutical precursor, p-coumaric acid,
from bacterial growth media at different time points during production.
The assay, extraction, and detection, performed on the LoD device,
proved to be a low cost and environmentally friendly approach, requiring
only a few tens of microliters of organic solvent and enabled detection
in a 3 μL volume. In addition, the data obtained from the centrifugal
platform showed a good correlation with data obtained from the high
performance liquid chromatography analysis.