Chen, Junjian Zhu, Yuchen Xiong, Menghua Hu, Guansong Zhan, Jiezhao Li, Tianjie Wang, Lin Wang, Yingjun Antimicrobial Titanium Surface via Click-Immobilization of Peptide and Its in Vitro/Vivo Activity The use of antimicrobial peptides (AMPs)-functionalized titanium implants is an efficient method for preventing bacterial infection. However, the attachment of AMPs to the surface of titanium implants remains a challenge. In this study, a “clickable” titanium surface was developed by using a silane coupling agent with an alkynyl group. The antimicrobial titanium implant was then constructed through the reaction between the “clickable” surface and azido-AMPs (PEG-HHC36:N<sub>3</sub>-PEG<sub>12</sub>-KRWWKWWRR) via click chemistry of Cu­(I)-catalyzed azide–alkyne cycloaddition (CuAAC). Such an antimicrobial titanium implant, with an AMP density of 897.4 ± 67.3 ng/cm<sup>2</sup> (2.5 ± 0.2 molecules per nm<sup>2</sup>) on the surface, exhibited good and stable antimicrobial activity, inhibited 90.2% of <i>Staphylococcus aureus</i> and 88.1% of <i>Escherichia coli</i> after 2.5 h of incubation, and even inhibited 69.5% of <i>Staphylococcus aureus</i> after 4 days of degradation. The CCK-8 assay indicated that the antimicrobial titanium implant exhibited negligible cytotoxicity to mouse bone mesenchymal stem cells. <i>In vivo</i> assay illustrated that this implant could kill 78.8% of <i>Staphylococcus aureus</i> after 7 days. This method has great potential for the preparation of antimicrobial titanium implants and the prevention of infections in the clinic. mouse bone mesenchymal;antimicrobial titanium implant;Antimicrobial Titanium Surface;AMP;PEG;antimicrobial titanium implants;Staphylococcus aureus;surface;CCK -8 assay;KRWWKWWRR;PEG-HHC 2018-12-07
    https://acs.figshare.com/articles/journal_contribution/Antimicrobial_Titanium_Surface_via_Click-Immobilization_of_Peptide_and_Its_in_Vitro_Vivo_Activity/7492553
10.1021/acsbiomaterials.8b01046.s001