%0 Journal Article
%A Chen, Junjian
%A Zhu, Yuchen
%A Xiong, Menghua
%A Hu, Guansong
%A Zhan, Jiezhao
%A Li, Tianjie
%A Wang, Lin
%A Wang, Yingjun
%D 2018
%T Antimicrobial Titanium Surface via Click-Immobilization
of Peptide and Its in Vitro/Vivo Activity
%U https://acs.figshare.com/articles/journal_contribution/Antimicrobial_Titanium_Surface_via_Click-Immobilization_of_Peptide_and_Its_in_Vitro_Vivo_Activity/7492553
%R 10.1021/acsbiomaterials.8b01046.s001
%2 https://acs.figshare.com/ndownloader/files/13879772
%K mouse bone mesenchymal
%K antimicrobial titanium implant
%K Antimicrobial Titanium Surface
%K AMP
%K PEG
%K antimicrobial titanium implants
%K Staphylococcus aureus
%K surface
%K CCK -8 assay
%K KRWWKWWRR
%K PEG-HHC
%X The
use of antimicrobial peptides (AMPs)-functionalized titanium
implants is an efficient method for preventing bacterial infection.
However, the attachment of AMPs to the surface of titanium implants
remains a challenge. In this study, a “clickable” titanium
surface was developed by using a silane coupling agent with an alkynyl
group. The antimicrobial titanium implant was then constructed through
the reaction between the “clickable” surface and azido-AMPs
(PEG-HHC36:N3-PEG12-KRWWKWWRR) via click chemistry
of Cu(I)-catalyzed azide–alkyne cycloaddition (CuAAC). Such
an antimicrobial titanium implant, with an AMP density of 897.4 ±
67.3 ng/cm2 (2.5 ± 0.2 molecules per nm2) on the surface, exhibited good and stable antimicrobial activity,
inhibited 90.2% of Staphylococcus aureus and 88.1%
of Escherichia coli after 2.5 h of incubation, and
even inhibited 69.5% of Staphylococcus aureus after
4 days of degradation. The CCK-8 assay indicated that the antimicrobial
titanium implant exhibited negligible cytotoxicity to mouse bone mesenchymal
stem cells. In vivo assay illustrated that this implant
could kill 78.8% of Staphylococcus aureus after 7
days. This method has great potential for the preparation of antimicrobial
titanium implants and the prevention of infections in the clinic.
%I ACS Publications