Zhou, Chuchao Liu, Shaokai Li, Jialun Guo, Ke Yuan, Quan Zhong, Aimei Yang, Jie Wang, Jiecong Sun, Jiaming Wang, Zhenxing Collagen Functionalized With Graphene Oxide Enhanced Biomimetic Mineralization and in Situ Bone Defect Repair Biomimetic mineralization using simulated body fluid (SBF) can form a bonelike apatite (Ap) on the natural polymers and enhance osteoconductivity and biocompatibility, and reduce immunological rejection. Nevertheless, the coating efficiency of the bonelike apatite layer on natural polymers still needs to be improved. Graphene oxide (GO) is rich in functional groups, such as carbonyls (−COOH) and hydroxyls (−OH), which can provide more active sites for biomimetic mineralization and improve the proliferation of the rat bone marrow stromal cells (r-BMSCs). In this study, we introduced 0%, 0.05%, 0.1%, and 0.2% w/v concentrations of GO into collagen (Col) scaffolds and immersed the fabricated scaffolds into SBF for 1, 7, and 14 days. In vitro environment scanning electron microscopy (ESEM), energy-dispersive spectrometry (EDS), thermogravimetric analysis (TGA), micro-CT, calcium quantitative analysis, and cellular analysis were used to evaluate the formation of bonelike apatite on the scaffolds. In vivo implantation of the scaffolds into the rat cranial defect was used to analyze the bone regeneration ability. The resulting GO–Col–Ap scaffolds exhibited a porous and interconnected structure coated with a homogeneous distribution of bonelike apatite on their surfaces. The Ca/P ratio of 0.1% GO–Col–Ap group was equal to that of natural bone tissue on the basis of EDS analysis. More apatites were observed in the 0.1% GO–Col–Ap group through TGA analysis, micro-CT evaluation, and calcium quantitative analysis. Furthermore, the 0.1% GO–Col–Ap group showed significantly higher r-BMSCs adhesion and proliferation in vitro and more than 2-fold higher bone formation than the Col–Ap group in vivo. Our study provides a new approach of introducing graphene oxide into bone tissue engineering scaffolds to enhance biomimetic mineralization. bone regeneration ability;rat cranial defect;Situ Bone Defect Repair Biomimetic mineralization;environment scanning electron microscopy;OH;rat bone marrow stromal cells;COOH;bonelike apatite;bonelike apatite layer;bone tissue engineering scaffolds;Graphene Oxide Enhanced Biomimetic Mineralization;EDS;SBF;TGA;biomimetic mineralization;analysis;ESEM 2018-11-26
    https://acs.figshare.com/articles/journal_contribution/Collagen_Functionalized_With_Graphene_Oxide_Enhanced_Biomimetic_Mineralization_and_in_Situ_Bone_Defect_Repair/7438775
10.1021/acsami.8b17636.s001