10.1021/acs.langmuir.8b02950.s002
A. Pasha Tabatabai
A. Pasha
Tabatabai
Benjamin P. Partlow
Benjamin P.
Partlow
Nicole R. Raia
Nicole R.
Raia
David L. Kaplan
David L.
Kaplan
Daniel L. Blair
Daniel L.
Blair
Silk Molecular
Weight Influences the Kinetics of Enzymatically Cross-linked Silk
Hydrogel Formation
American Chemical Society
2018
silk Molecular Weight Influences
reconstituted silk solutions
bulk rheological properties
weight changes
covalent dityrosine cross-linking
Enzymatically Cross-linked Silk Hydrogel Formation
protein
hydrogel
time-resolved bond formation
mechanism
polymerization
gelation process
growth postpercolation
covalent dityrosine bond formation
polymerize
model
mode
Kinetic
2018-11-13 00:00:00
Journal contribution
https://acs.figshare.com/articles/journal_contribution/Silk_Molecular_Weight_Influences_the_Kinetics_of_Enzymatically_Cross-linked_Silk_Hydrogel_Formation/7388681
We transform reconstituted silk solutions into robust hydrogels through covalent
dityrosine cross-linking resulting from an enzymatic reaction. The
bulk rheological properties and the covalent dityrosine bond formation
of these gels are measured during polymerization. We compare the time-resolved
bond formation to the mechanical properties, where we find that the
gelation process is consistent with a model of percolation. The molecular
weight of the protein determines whether a secondary mode of growth
postpercolation exists, indicating that molecular weight changes affect
the mechanisms by which these gels polymerize.