Impact
of Morphology on Iron Oxide Nanoparticles-Induced
Inflammasome Activation in Macrophages
Liu Liu
Rui Sha
Lijiao Yang
Xiaomin Zhao
Yangyang Zhu
Jinhao Gao
Yunjiao Zhang
Long-Ping Wen
10.1021/acsami.8b17474.s001
https://acs.figshare.com/articles/journal_contribution/Impact_of_Morphology_on_Iron_Oxide_Nanoparticles-Induced_Inflammasome_Activation_in_Macrophages/7373885
Inflammasomes,
a critical component of the innate immune system,
mediate much of the inflammatory response manifested by engineered
nanomaterials. Iron oxide nanoparticles (IONPs), a type of nanoparticles
that have gained widespread acceptance in preclinical and clinical
settings, are known to induce inflammasome activation, but how morphology
affects the inflammasome-activating property of IONPs has not been
addressed. In this report, we have synthesized four morphologically
distinct IONPs having the same aspect ratio and similar surface charge,
thus offering an ideal system to assess the impact of morphology on
nanoparticle-elicited biological effect. We show that morphology was
a critical determinant for IONP-induced IL-1β release and pyroptosis,
with the octapod and plate IONPs exhibiting significantly higher activity
than the cube and sphere IONPs. The inflammasome-activating capacity
of different IONPs correlated with their respective ability to elicit
intracellular reactive oxygen species generation, lysosomal damage,
and potassium efflux, three well-known mechanisms for nanoparticle-facilitated
inflammasome activation. Furthermore, we demonstrate that the release
of IL-1β induced by IONPs was only partly mediated by NLRP3,
suggesting that inflammasomes other than NLRP3 are also involved in IONP-induced inflammasome
activation. Our results may have implications for designing safer
nanoparticles for in vivo applications.
2018-11-06 00:00:00
morphology
IONP-induced IL -1β release
Macrophages Inflammasomes
Iron Oxide Nanoparticles-Induced Inflammasome Activation
inflammasome-activating capacity
vivo applications
IL -1β
nanoparticle-facilitated inflammasome activation
inflammasome-activating property
surface charge
iron oxide nanoparticles
plate IONPs
intracellular reactive oxygen species generation
IONP-induced inflammasome activation
NLRP 3
lysosomal damage
sphere IONPs
potassium efflux
aspect ratio
inflammasome activation