Fan, Yao-Yao Mou, Zhao-Li Wang, Man Li, Jun Zhang, Jing Dang, Fu-Quan Zhang, Zhi-Qi Chimeric Aptamers-Based and MoS<sub>2</sub> Nanosheet-Enhanced Label-Free Fluorescence Polarization Strategy for Adenosine Triphosphate Detection Adenosine triphosphate (ATP) as a primary energy source plays a unique role in the regulation of all cellular events. The necessity to detect ATP requires sensitive and accurate quantitative analytical strategies. Herein, we present our study of developing a MoS<sub>2</sub> nanosheet-enhanced aptasensor for fluorescence polarization-based ATP detection. A bifunctional DNA strand was designed to consist of chimeric aptamers that recognize and capture ATP and berberine, a fluorescence enhancer. In the absence of ATP, the DNA strand bound to berberine will be hydrolyzed when Exonuclease I (Exo I) is introduced, releasing berberine as a result. In contrast, when ATP is present, ATP aptamer folds into a G-quadruplex structure; thus, the complex can resist degradation by Exo I to maintain berberine for fluorescent detection purpose. In addition, to magnify the fluorescence polarization (FP) signal, MoS<sub>2</sub> nanosheets were also adopted in the system. This nanosheets-enhanced FP strategy is simple and facile which does not require traditional dye-labeled DNA strands and complex operation steps. The developed fluorescence polarization aptasensor showed high sensitivity for the quantification of ATP with a detection limit of 34.4 nM, performing well both in buffer solution and in biological samples. MoS 2 nanosheets;ATP aptamer folds;berberine;34.4 nM;DNA strand;operation steps;fluorescence polarization-based ATP detection;bifunctional DNA strand;fluorescence polarization;G-quadruplex structure;detection purpose;fluorescence polarization aptasensor;nanosheets-enhanced FP strategy;MoS 2 nanosheet-enhanced aptasensor;chimeric aptamers;fluorescence enhancer;Chimeric Aptamers-Based;energy source;Adenosine Triphosphate Detection Adenosine triphosphate;detection limit;buffer solution;dye-labeled DNA strands;MoS 2 Nanosheet-Enhanced Label-Free Fluorescence Polarization Strategy 2018-10-23
    https://acs.figshare.com/articles/journal_contribution/Chimeric_Aptamers-Based_and_MoS_sub_2_sub_Nanosheet-Enhanced_Label-Free_Fluorescence_Polarization_Strategy_for_Adenosine_Triphosphate_Detection/7289459
10.1021/acs.analchem.8b04107.s001