Visualizing the Solid–Liquid Interface of Conjugated Copolymer Films Using Fluorescent Liposomes Yi Zhang Achilleas Savva Shofarul Wustoni Adel Hama Iuliana P. Maria Alexander Giovannitti Iain McCulloch Sahika Inal 10.1021/acsabm.8b00323.s001 https://acs.figshare.com/articles/journal_contribution/Visualizing_the_Solid_Liquid_Interface_of_Conjugated_Copolymer_Films_Using_Fluorescent_Liposomes/7252505 Conjugated polymers are promising engineering tools for establishing bilateral electrical communication with living systems. The free energy of their films, the roughness, and charge density play major roles in determining their interactions with lipid bilayers, which form the membrane barrier around every living cell allowing for molecular exchange with the extracellular environment. In this work, we investigate lipid bilayer formation from synthetic lipid vesicles (liposomes) on a series of amphiphilic copolymer films based on naphthalene 1,4,5,8 tetracarboxylic diimide bithiophene (NDI-T2) backbone where the alkyl side chain is gradually exchanged for an ethylene glycol-based side chain. As the concentration of ethylene glycol in the composition changes, the surface energy of the films varies drastically, which, in turn, effects the interactions with liposomes. By imaging the interactions of fluorophore-labeled liposomes with these surfaces via a fluorescence microscope, we show that the films can be cast such that ethylene glycol-rich regions, which liposomes favor, are accumulated on the surface and extract information on the wettability behavior that has not been possible using other surface sensitive techniques. This approach uncovers the solid/liquid interface of a promising class of electron transporting conjugated polymer films and suggests synthetic strategies to maximize the number of lipid-polymer contacts for the formation of supported lipid bilayers. 2018-10-15 00:00:00 liposomes NDI-T lipid bilayers interaction Fluorescent Liposomes Conjugated polymers Conjugated Copolymer Films alkyl side chain ethylene glycol-based side chain lipid bilayer formation surface ethylene glycol-rich regions amphiphilic copolymer films