Roman, Jean Français, Olivier Jarroux, Nathalie Patriarche, Gilles Pelta, Juan Bacri, Laurent Pioufle, Bruno Le Solid-State Nanopore Easy Chip Integration in a Cheap and Reusable Microfluidic Device for Ion Transport and Polymer Conformation Sensing Solid-state nanopores have a huge potential in upcoming societal challenging applications in biotechnologies, environment, health, and energy. Nowadays, these sensors are often used within bulky fluidic devices that can cause cross-contaminations and risky nanopore chips manipulations, leading to a short experimental lifetime. We describe the easy, fast, and cheap innovative 3D-printer-helped protocol to manufacture a microfluidic device permitting the reversible integration of a silicon based chip containing a single nanopore. We show the relevance of the shape of the obtained channels thanks to finite elements simulations. We use this device to thoroughly investigate the ionic transport through the solid-state nanopore as a function of applied voltage, salt nature, and concentration. Furthermore, its reliability is proved through the characterization of a polymer-based model of protein–urea interactions on the nanometric scale thanks to a hairy nanopore. Solid-state nanopores;3 D-printer-helped protocol;polymer-based model;microfluidic device;Solid-State Nanopore Easy Chip Integration;Reusable Microfluidic Device;Ion Transport;fluidic devices;nanometric scale thanks;channels thanks;Polymer Conformation;salt nature;elements simulations;nanopore chips manipulations 2018-10-04
    https://acs.figshare.com/articles/journal_contribution/Solid-State_Nanopore_Easy_Chip_Integration_in_a_Cheap_and_Reusable_Microfluidic_Device_for_Ion_Transport_and_Polymer_Conformation_Sensing/7215215
10.1021/acssensors.8b00700.s001