E‑Beam
Nanostructuring and Direct Click Biofunctionalization
of Thiol–Ene Resist
Reza Zandi Shafagh
Alexander Vastesson
Weijin Guo
Wouter van der Wijngaart
Tommy Haraldsson
10.1021/acsnano.8b03709.s001
https://acs.figshare.com/articles/journal_contribution/E_Beam_Nanostructuring_and_Direct_Click_Biofunctionalization_of_Thiol_Ene_Resist/7098302
Electron
beam lithography (EBL) is of major importance for ultraminiaturized
biohybrid system fabrication, as it allows combining biomolecular
patterning and mechanical structure definition on the nanoscale. Existing
methods are limited by multistep biomolecule immobilization procedures,
harsh processing conditions that are harmful to sensitive biomolecules,
or the structural properties of the resulting protein monolayers or
hydrogel-based resists. This work introduces a thiol–ene EBL
resist with chemically reactive thiol groups on its native surface
that allow the direct and selective “click” immobilization
of biomolecules under benign processing conditions. We constructed
EBL structured features of size down to 20 nm, and direct functionalized
the nanostructures with a sandwich of biotin and streptavidin. The
facile combination of polymer nanostructuring with biomolecule immobilization
enables mechanically robust biohybrid components of interest for nanoscale
biomedical, electronic, photonic, and robotic applications.
2018-09-13 00:00:00
reactive thiol groups
Electron beam lithography
nanoscale
ultraminiaturized biohybrid system fabrication
EBL
processing conditions
multistep biomolecule immobilization procedures
Direct Click Biofunctionalization