10.1021/acsbiomaterials.8b00521.s001
Alejandro Garcia Garcia
Alejandro
Garcia Garcia
Anne Hébraud
Anne
Hébraud
Jean-Luc Duval
Jean-Luc
Duval
Corinne R. Wittmer
Corinne R.
Wittmer
Ludovic Gaut
Ludovic
Gaut
Delphine Duprez
Delphine
Duprez
Christophe Egles
Christophe
Egles
Fahmi Bedoui
Fahmi
Bedoui
Guy Schlatter
Guy
Schlatter
Cecile Legallais
Cecile
Legallais
Poly(ε-caprolactone)/Hydroxyapatite 3D Honeycomb
Scaffolds for a Cellular Microenvironment Adapted to Maxillofacial
Bone Reconstruction
American Chemical Society
2018
differentiation
tissue engineering approaches
PCL-HA honeycomb structure
material biomechanical strength
scaffold
bone cells
bone reconstruction
Maxillofacial Bone Reconstruction
RNA
3 D environment
160 μ m
2 D culture
PCL-HA honeycomb structures
2018-08-20 00:00:00
Journal contribution
https://acs.figshare.com/articles/journal_contribution/Poly_-caprolactone_Hydroxyapatite_3D_Honeycomb_Scaffolds_for_a_Cellular_Microenvironment_Adapted_to_Maxillofacial_Bone_Reconstruction/7015325
The elaboration of
biomimetic materials inspired from the specific
structure of native bone is one the main goal of tissue engineering
approaches. To offer the most appropriate environment for bone reconstruction,
we combined electrospinning and electrospraying to elaborate an innovative
scaffold composed of alternating layers of polycaprolactone (PCL)
and hydroxyapatite (HA). In our approach, the electrospun PCL was
shaped into a honeycomb-like structure with an inner diameter of 160
μm, capable of providing bone cells with a 3D environment while
ensuring the material biomechanical strength. After 5 days of culture
without any differentiation factor, the murine embryonic cell line
demonstrated excellent cell viability on contact with the PCL-HA structures
as well as active colonization of the scaffold. The cell differentiation,
as tested by RT-qPCR, revealed a 6-fold increase in the expression
of the RNA of the Bglap involved in bone mineralization as compared
to a classical 2D culture. This differentiation of the cells into
osteoblasts was confirmed by alkaline phosphatase staining of the
scaffold cultivated with the cell lineage. Later on, organotypic cultures
of embryonic bone tissues showed the high capacity of the PCL-HA honeycomb
structure to guide the migration of differentiated bone cells throughout
the cavities and the ridge of the biomaterial, with a colonization
surface twice as big as that of the control. Taken together, our results
indicate that PCL-HA honeycomb structures are biomimetic supports
that promotes in vitro osteocompatibility, osteoconduction, and osteoinduction
and could be suitable for being used for bone reconstruction in complex
situations such as the repair of maxillofacial defects.