10.1021/acs.jpcc.8b06117.s001 Raju R. Kumal Raju R. Kumal Mohammad Abu-Laban Mohammad Abu-Laban Prakash Hamal Prakash Hamal Blake Kruger Blake Kruger Holden T. Smith Holden T. Smith Daniel J. Hayes Daniel J. Hayes Louis H. Haber Louis H. Haber Near-Infrared Photothermal Release of siRNA from the Surface of Colloidal Gold–Silver–Gold Core–Shell–Shell Nanoparticles Studied with Second-Harmonic Generation American Chemical Society 2018 CSS nanoparticles photothermal cleaving kinetics NIR laser powers photothermal cleaving linkers SHG Second-Harmonic Generation Photothermal release plasmonic CSS nanoparticles plasmonic nanoparticles Near-Infrared Photothermal Release photothermal cleaving surface-sensitive time-dependent second-harmonic generation GNP nanoparticle-based NIR-mediated gold nanoparticles bind siRNA photothermal release drug delivery nanoparticle surface 2018-08-06 00:00:00 Journal contribution https://acs.figshare.com/articles/journal_contribution/Near-Infrared_Photothermal_Release_of_siRNA_from_the_Surface_of_Colloidal_Gold_Silver_Gold_Core_Shell_Shell_Nanoparticles_Studied_with_Second-Harmonic_Generation/6981536 Photothermal release of oligonucleotides from the surface of plasmonic nanoparticles represents a promising platform for spatiotemporal controlled drug delivery. Here we demonstrate the use of novel gold–silver–gold core–shell–shell (CSS) nanoparticles to study the photothermal cleaving and release of micro-RNA (miRNA) mimics or small interfering RNA (siRNA) under near-infrared (NIR) irradiation. The furan–maleimide-based Diels–Alder adduct cleaves thermally above 60 °C and is used to bind siRNA to the colloidal nanoparticle surface in water. We investigate the photothermal cleaving kinetics of siRNA under different NIR laser powers using surface-sensitive time-dependent second-harmonic generation (SHG) spectroscopy. The photothermal release of siRNA from the surface of CSS nanoparticles is significantly higher than that from the surface of gold nanoparticles (GNPs) under similar experimental conditions. These results demonstrate that plasmonic CSS nanoparticles with photothermal cleaving linkers have important potential applications for nanoparticle-based NIR-mediated drug-delivery systems.